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COMPARATIVE DISTRIBUTION OF SUCCINIC DEHYDROGENASE IN SIX MAMMALS AND MODIFICATION IN THE HISTOCHEMICAL TECHNIC

ALEXANDER M. RUTENBURG 1, MOSHE WOLMAN 1, and ARNOLD M. SELIGMAN 1

1 Yamins Laboratory for Surgical Research, Beth Israel Hospital, and Department of Surgery, Harvard Medical School, Boston

The distribution of succinic dehydrogenase activity was compared in the organs of six mammals. The histochemical localization of the enzyme was improved so as to enable the use of thin frozen sections (10 µ) by the addition of the following ions:bicarbonate, calcium, magnesium and aluminum. The distribution of enzymatic activity by this technic in the organs of the rat was compared with the earlier method.


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