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PRECIPITATION OF CALCIUM PHOSPHATE IN THE HISTOCHEMICAL METHOD FOR PHOSPHATASE

GEORGE GOMORI 1 and EARL P. BENDITT 1

1 From the Departments of Medicine and of Pathology, The University of Chicago, Chicago, Ill.

Ca phosphate, when precipitated by the addition of phosphate to solutions similar in composition to the histochemical substrate mixture, does not show any experimentally demonstrable tendency to supersaturation. The factors which may contribute to false localizations of enzymatic activity its the Ca-CoS method for alkaline phosphatase are not well understood and certainly not amenable to mathematical analysis for the time being. At present, diffusion artifacts do not appear to be an important source of error, provided incubation time is not unduly extended.

Submitted on November 28, 1952


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