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THE FERRIC FERRICYANIDE REDUCTION TEST IN HISTOCHEMISTRY

R. D. LILLIE 1 and HELEN J. BURTNER 1

1 National Institutes of Health, Bethesda 14, Md.

Ascorbic, oxalic and uric acids, phenols, indols, pyrrols, aryl amines, hydrazines, thiols and inorganic sulfides and peroxide promptly reduce ferric ferricyanide mixtures to Prussian blue. Most of the other substances tested reacted feebly or slowly, and probably need not be considered in the histochemical test, provided the reaction time is kept down to 10 to 15 minutes.

Submitted on October 31, 1952


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