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A METHOD FOR RETAINING BACTERIAL CELLS IN PLACE FOR STAINING AND CYTOCHEMICAL TESTS FOR LIGHT OR ELECTRON MICROSCOPY

NORMAN C. DONDERO 1 and M. R. ZELLE 1

1 Cornell University, Laboratory of Bacteriology, Ithaca, New York

A method of retaining bacterial cells in place on glass cover slips or on electron microscope films for cytochemical tests was described. The method consisted basically of applying a film of "formvar" over the cells on cover slips or on electron microscope object-supporting screens. The "formvar" film did not interfere with many stains and cytochemical tests, nor with the use of enzymes.

Submitted on June 18, 1953


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