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THE QUANTITATIVE HISTOCHEMISTRY OF THE BRAIN : HISTOLOGICAL SAMPLING
1 Department of Pharmacology, Washington University Medical School, St. Louis
The general histochemical procedure of Linderstrøm-Lang and Holter has been altered to permit direct histological control and the use of smaller samples. Frozen sections are dried at –30°C. From the dry sections are cut out identified regions as small as 100 x 100 x 20µ (0.2
wet weight). The chemical analysis of these fragments may be based on either their protein content, their dry weight or their volume. The weight and volume measurements are described and validated. The revised technique is not limited in applicability to the nervous system.
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