Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by DEIERKAUF, F. A.
Right arrow Articles by HESLINGA, F. J. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by DEIERKAUF, F. A.
Right arrow Articles by HESLINGA, F. J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

THE ACTION OF FORMALDEHYDE ON RAT BRAIN LIPIDS

F. A. DEIERKAUF 1 and F. J. M. HESLINGA 1

1 Department of Medical Chemistry, University of Leyden, Holland

The influence of formaldehyde on rat brain lipid was investigated by comparing the analysis of lipids from fresh rat brain with the analysis from rat brains fixed for 110 days in a formaldehyde solution.

A quantitative analysis of the lipids using column and paper chromatography is described.

Sphingomyclin, cerebrosides and sulphatides were not attacked. Only a slight diminution of phosphatidylserine was indicated. Phosphoinositides remain fairly constant. Of lecithin about 25% was converted to lyso-lecithin. The phosphatidylethanolamine and lyso-phosphatidylethanolamine fractions suffered a marked diminution and the reaction with formaldehyde was noticeable in a change of chromatographic behaviour. The fraction containing cholesterol, cholesterol esters, glycerides, and fatty acids increased, the increase being correlated with the decrease of other fractions by the formation of lyso compounds.

After storing tissues for months in formaldehyde solutions a reliable analysis of the lipid content is still possible.

Submitted on June 30, 1961


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1962