|
|
|
|
 |
|||
|
|||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE
1 Department of Bacteriology and Immunology, Harvard Medical School, Boston, Mass.
A method is described for the fixation of blocks of tissue for use in studies employing immunofluorescence. This method consists of fixing thin blocks in 95% ethanol and carrying out the subsequent dehydration and clearing at refrigerator temperatures (4°C). Thereafter, embedding in paraffin and sectioning by the standard microtomy is easy.
This method results in preparations which are histologically more precise in the localization of antigen or antibody than preparations of frozen tissues; and with rabbit antibody and bovine serum albumin, the sensitivity of detection is enhanced. Bovine serum albumin can be found for longer periods after injection than is possible with frozen sections.
Other antigens for which this procedure has proved satisfactory are bovine gamma globulin, horse ferritin, influenza A virus, diphtheria and tetanus toxoids. Hen's ovalbumin deteriorated.
New antigens or new antibodies should be tested before being committed to this method.
Submitted on July 24, 1961
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. de Moreno de LeBlanc, J. G. LeBlanc, G. Perdigon, A. Miyoshi, P. Langella, V. Azevedo, and F. Sesma Oral administration of a catalase-producing Lactococcus lactis can prevent a chemically induced colon cancer in mice J. Med. Microbiol., January 1, 2008; 57(1): 100 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sollwedel, A. Z. Bertoja, M. L. Zenclussen, K. Gerlof, U. Lisewski, P. Wafula, B. Sawitzki, C. Woiciechowsky, H.-D. Volk, and A. C. Zenclussen Protection from Abortion by Heme Oxygenase-1 Up-Regulation Is Associated with Increased Levels of Bag-1 and Neuropilin-1 at the Fetal-Maternal Interface J. Immunol., October 15, 2005; 175(8): 4875 - 4885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Alignani, B. Maletto, M. Liscovsky, A. Ropolo, G. Moron, and M. C. Pistoresi-Palencia Orally administered OVA/CpG-ODN induces specific mucosal and systemic immune response in young and aged mice J. Leukoc. Biol., June 1, 2005; 77(6): 898 - 905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hammiche, M. J. German, R. Hewitt, H. M. Pollock, and F. L. Martin Monitoring Cell Cycle Distributions in MCF-7 Cells Using Near-Field Photothermal Microspectroscopy Biophys. J., May 1, 2005; 88(5): 3699 - 3706. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Guérin-Danan, J.-C. Meslin, A. Chambard, A. Charpilienne, P. Relano, C. Bouley, J. Cohen, and C. Andrieux Food Supplementation with Milk Fermented by Lactobacillus casei DN-114 001 Protects Suckling Rats from Rotavirus-Associated Diarrhea J. Nutr., January 1, 2001; 131(1): 111 - 117. [Abstract] [Full Text]
|
|
|
|
 |
|
 S N Andersen, T Lovig, O P F Clausen, A Bakka, O Fausa, and T O Rognum Villous, hypermucinous mucosa in long standing ulcerative colitis shows high frequency of K-ras mutations Gut, November 1, 1999; 45(5): 686 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Aspberg, S. Adam, G. Kostka, R. Timpl, and D. Heinegard Fibulin-1 Is a Ligand for the C-type Lectin Domains of Aggrecan and Versican J. Biol. Chem., July 16, 1999; 274(29): 20444 - 20449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Donze, J. E. Cummins Jr., R. S. Schwiebert, P. N. Fultz, S. Jackson, and J. Mestecky Human and Nonhuman Primate Lymphocytes Engrafted into SCID Mice Reside in Unique Mesenteric Lymphoid Structures J. Immunol., August 1, 1998; 161(3): 1306 - 1312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. H. Donze, J. E. Cummins Jr., R. S. Schwiebert, A. Kantele, Y. Han, P. N. Fultz, S. Jackson, and J. Mestecky HIV-1/Simian Immunodeficiency Virus Infection of Human and Nonhuman Primate Lymphocytes Results in the Migration of CD2+ T Cells into the Intestine of Engrafted SCID Mice J. Immunol., March 1, 1998; 160(5): 2506 - 2513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sunyer, L. Rothe, D. Kirsch, X. Jiang, F. Anderson, P. Osdoby, and P. Collin-Osdoby Ca2+ or Phorbol Ester But Not Inflammatory Stimuli Elevate Inducible Nitric Oxide Synthase Messenger Ribonucleic Acid and Nitric Oxide (NO) Release in Avian Osteoclasts: Autocrine NO Mediates Ca2+-Inhibited Bone Resorption Endocrinology, May 1, 1997; 138(5): 2148 - 2162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Czekay, R. Orlando, L Woodward, E. Adamson, and M. Farquhar The expression of megalin (gp330) and LRP diverges during F9 cell differentiation J. Cell Sci., January 4, 1995; 108(4): 1433 - 1441. [Abstract] [PDF]
|
|
|
|
|
|
H. Fukamachi Proliferation and differentiation of fetal rat intestinal epithelial cells in primary serum-free culture J. Cell Sci., October 1, 1992; 103(2): 511 - 519. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Yoo, Y Yazawa, K Tomoda, and R Floyd Type II collagen-induced autoimmune endolymphatic hydrops in guinea pig Science, October 7, 1983; 222(4619): 65 - 67. [Abstract] [PDF]
|
|
|
|
|
|
A. M. Johnson, C. A. Alper, F. S. Rosen, and J. M. Craig C1 Inhibitor: Evidence for Decreased Hepatic Synthesis in Hereditary Angioneurotic Edema Science, August 6, 1971; 173(3996): 553 - 554. [Abstract] [PDF]
|
|
|
|
|
|
J. H. Peters Heterophile Reactive Antigen in Infectious Mononucleosis Science, September 8, 1967; 157(3793): 1200 - 1202. [Abstract] [PDF]
|
|
|
|
|
|
C. Takata, J. F. Albright, and T. Yamada Lens Fiber Differentiation and Gamma Crystallins: Immunofluorescent Study of Wolffian Regeneration Science, March 12, 1965; 147(3663): 1299 - 1301. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |