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AN AUTORADIOGRAPHIC STUDY OF THE LOCALIZATION AND DISTRIBUTION OF TRITIATED HISTIDINE IN BONE

E. A. TONNA 1, E. P. CRONKITE 1, and M. PAVELEC 1

1 Medical Research Center, Brookhaven National Laboratory, Upton, N. Y.

The localization, distribution and cellular turnover of tritium labeled-histidine was followed autoradiographically in the femora of young mice. Initially the labeled amino acid was taken up by the cellular phase of the skeletal system within the first hour after isotope administration. From 1 to 4 hours, intracellular histidine appeared within newly deposited cartilage and bone matrix. By 14 days skeletal cells had lost their label, however, red blood cells were still labeled at 14 days.

Submitted on March 8, 1962


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