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ELECTRO- AND IMMUNOELECTROPHORETIC PATTERNS IN THE LARVAL SALIVARY SECRETION OF CHIRONOMUS THUMMI

K.-I. KATO 1, E. PERKOWSKA 1, and J. L. SIRLIN 1

1 Institute of Animal Genetics, Edinburgh, Scotland

The salivary secretion of chironomid larvae was studied electro- and immunoelectrophoretically.

The secretion could be resolved into 14 nigrosin-positive (protein) bands and 10 metachromatic (mucopolysaccharide) bands in the pherograms. Much less stainability was found in some of these bands in the pherograms of the gland cell components. The polysaccharide bands roughly corresponded with an equal number of protein bands at the same positions in the pherogram.

An antiserum against a cell extract of whole larvae (excluding the salivary glands) yielded 6 groups of precipitation crescents on the pherogram of the cell extract. The same antiserum which had been previously absorbed with the salivary secretion yields a reduced number of crescents, specially on the anodic side of the pherogram.

Submitted on November 20, 1962


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