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THE COMBINED USE OF MICROINCINERATION AND THE PRUSSIAN BLUE REACTION FOR A MORE SENSITIVE HISTOCHEMICAL DEMONSTRATION OF IRON

R. H. FENTON 1, F. B. JOHNSON 1, and L. E. ZIMMERMAN 1

1 Ophthalmic Pathology and Histochemistry Branches, Armed Forces Institute of Pathology, Washington 25, D. C.

The Prussian blue reaction was found useful for the demonstration of iron in the celloidinized ash left after direct microincineration. This method has been suggested previously, although it has not become popular. It is a very sensitive means of demonstrating the presence of iron in tissues and has application in cases in which the quantities of iron are very small or in which the iron is in a protein-bound form that fails to give a positive reaction with the usual Prussian blue technique.

Submitted on May 22, 1963


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