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HISTOCHEMICAL RECOGNITION OF CALCIUM OXALATE

PHILIP PIZZOLATO 1

1 Clinical Laboratory, Veterans Administration Hospital, and Department of Pathology, Louisiana State University, School of Medicine, New Orleans

Of the workers applying the von Kóssa test to calcium oxalate crystals in animal tissue, about half reported blackening in silver nitrate on exposure to light. We found that "pure" calcium oxalate crystals, produced in rats by the administration of ammonium oxalate or ethylene glycol, gave a negative von Kóssa reaction. These crystals, however, were blackened when treated with a solution of silver nitrate in concentrated hydrogen peroxide. Other von Kóssa negative salts, such as CaF2 or CaSO4·2H2O, do not respond to the H2O2 and AgNO3 test.

Submitted on September 12, 1963


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