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CYTOCHEMISTRY OF PARACOCCIDIOIDES BRASILIENSIS. I. CYTOCHEMISTRY OF CYTOPLASMIC POLYSACCHARIDES IN YEAST FORM CULTURES WITH LIGHT MICROSCOPE

LUIS M. CARBONELL 1, HAYDEE CASTEJON 1, and LADISLAO POLLAK 1

1 Department of Experimental Pathology, Instituto Venezolano de Investigaciones Cientificas, Apartado 1827, Caracas and Instituto Nacional de Tuberculosis, Caracas, Venezuela

1. The histochemical distribution and localization of cytoplasmic polysaccharides are studied in the yeast form of P. brasiliensis. Glycogen and an acid mucopolysaccharide probably containing sulfate groups are demonstrated. These polysaccharides disappear after the sixth day of culture. The glycogen and the acid mucopolysaccharide are bound to protein.

2. Stains and enzyme treatments applied in sequence help demonstrate the site of different polysaccharides.

Submitted on October 14, 1963


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