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SOME ASPECTS OF THE SPECIFICITY OF FATTY ACID ESTERASES

B. H. J. HOFSTEE 1

1 Palo Alto Medical Research Foundation, Palo Alto, California

A comparison has been made of the meaning of the experimental kinetic constants Vm, KM and Vm/KM with respect to group transfer by a hydrolase proceeding either with or without obligatory intermediary transfer of the group to the enzyme.

The results have been applied to the interpretation of kinetic data in a number of actual cases involving esterases whereby either several substrates have one of these experimental constants in common or the addition of a modifier has no effect on one of these constants with respect to a particular substrate.

Submitted on March 23, 1964


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