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HISTOCHEMISTRY OF TISSUE CULTURE CELLS: A STUDY OF THE EFFECTS OF FREEZING AND OF SOME FIXATIVES

V. MAGGI 1 and P. N. RIDDLE 1

1 Tissue and Organ Culture Unit, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, W.C.2.

It has been shown that a) the membranes of HeLa and L cell lysosomes are readily permeable to naphthol AS-AM phosphate when used as a substrate for acid phosphatase, b) freezing as well as fixation affect the permeability of the lysosomal membrane to both naphthol AS-AM phosphate and beta-glycerophosphate, c) none of these treatments seem to affect greatly the plasma or the mitochondrial membranes and d) the distribution of phospholipids in the cells is also affected by freezing or fixation.

Submitted on October 26, 1964


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