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ALDEHYDE-FUCHSIN STAINING APPLIED TO FROZEN SECTIONS FOR DEMONSTRATING PITUITARY AND PANCREATIC BETA CELLS

BARBARA M. JENNINGS 1

1 Department of Pathology, University of California School of Medicine, San Francisco, California

Aldehyde-fuchsin staining was applied to fresh frozen sections to frozen sections pre-fixed with sucrose-formol, and to freeze-dried sections of human and animal pancreas and pituitary, with positive reactions in beta-cells of both tissues. Successful adaptation of aldehyde-fuchsin staining to this material was achieved largely by choice of fixative, use of a fresh, nonchilled solution of aldehyde-fuchsin, and by acidified permanganate oxidation.

Certain standard fixatives (formol, Zenker-formol, and Bouin) proved satisfactory, as well as a more recently introduced one, glutaraldehyde. A fixation-staining technique requiring less than 10 minutes was evolved using stock (25%) glutaraldehyde.

With some of these fixatives, inserting an aldehyde blockade step when necessary, the Periodic and Schiff reaction could be employed as a control stain for aldehyde-fuchsin, with comparable results.

The methods adopted are useful in identifying beta-cells in surgical specimens and in material used for enzyme reactions and fluorescent antibody techniques.

Submitted on November 16, 1964


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