Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ANDROS, G.
Right arrow Articles by WOLLMAN, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ANDROS, G.
Right arrow Articles by WOLLMAN, S. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

AUTORADIOGRAPHY OF DIFFUSIBLE IONS WITH APPLICATION TO THYROIDAL RADIOIODIDE

GEORGE ANDROS 1 and SEYMOUR H. WOLLMAN 1

1 Laboratory of Physiology, National Cancer Institute, National Institues of Health, Bethesda, Maryland

A method for the autoradiographic localization of water-soluble diffusible ions has been developed and applied to the localization of iodide125 in the mouse thyroid gland. Frozen-dried thyroids of hypophysectomized mice containing radioiodine125 as inorganic iodide125 were embedded in paraffin. Sections were spread on mercury and mounted on autoradiographic film. After exposure the mounted section was permanently fastened directly over its latent image and tissue constituents were fixed by dipping in 80% aqueous methanol. The preparation was air dried and then deparaffinized with petroleum either. After development and fixation, slides were stained with Nuclear Fast Red. In certain cases radioiodide could be localized primarily in the follicular epithelium. Control studies demonstrated that the autoradiographic image was not an artifact resulting from chemical induction or suppression of selected areas of latent image. A comparison of autoradiographs prepared from frozen sections and those prepared by freeze-drying showed similar localization of the radioisotope and indicated that there was no displacement of the radioiodide during paraffin embedding.

Submitted on December 20, 1964


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1965