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ON THE IDENTITY OF "NOTHING DEHYDROGENASE"

CHARLES R. SHAW 1 and ANN L. KOEN 1

1 Biological Research Unit, Hawthorn Center, Northville, Michigan

The histochemical demonstration of dehydrogenase activity using tetrazolium sometimes occurs in the absence of added substrate. This occurrence has been called "nothing dehydrogenase". The present study, based on starch gel electrophoresis of mouse tissues and of pure alcohol dehydrogenase, as well as quantitative measurements of the latter, indicates that alcohol dehydrogenase is the major agent of "nothing dehydrogenase" activity.

Submitted on December 17, 1964


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R. K. Selander and S. Y. Yang
Horseshoe Crab Lactate Dehydrogenases: Evidence for Dimeric Structure
Science, July 10, 1970; 169(3941): 179 - 181.
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E. J. Massaro
Horseshoe Crab Lactate Dehydrogenase: Tissue Distribution and Molecular Weight
Science, February 13, 1970; 167(3920): 994 - 996.
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E. Beutler, C. R. Shaw, A. L. Koen, P. Cuatrecasas, and S. Segal
"Galactose Dehydrogenase," "Nothing Dehydrogenase," and Alcohol Dehydrogenase: Interrelation
Science, June 16, 1967; 156(3781): 1516 - 1518.
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