Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HASHIMOTO, T.
Right arrow Articles by BURSTONE, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HASHIMOTO, T.
Right arrow Articles by BURSTONE, M. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

DISTRIBUTION PATTERNS OF OXIDATIVE ENZYMES IN EXPERIMENTALLY INDUCED TUMORS, REGENERATION AND INFLAMMATION OF MOUSE SKIN

TAKENORI HASHIMOTO 1 and M. S. BURSTONE 1

1 National Cancer Institute, National Institutes of Health, Bethesda, Maryland

Enzyme histochemical studies of normal and burned mouse skin as well as those treated with methylcholanthrene or 2,4-dinitro-1-chlorobenzene revealed that the irritant (or stimulus) carcinogenic or noncarcinogenic, initially results in a more distinct polarization2 of the localization of oxidative enzymes including glucose 6-phosphate dehydrogenase (predominant in the upper layers of the hypertrophic epidermis) and DPN-dependent dehydrogenases, succinic dehydrogenase and cytochrome oxidase in the lower layers. Subsequently, only the carcinogen disrupted the polarization pattern. Methylcholanthrene-induced squamous cell carcinomas were classified from a histochemical standpoint according to the following three types: (1) those well differentiated and having a distinctly polarized pattern of oxidative enzymes; (2) those showing disruption of the polarization pattern and marked activity of oxidative enzymes; and (3) those that were undifferentiated and characterized by absence of a polarized pattern and weak activity of oxidative enzymes. Type 1 showed an enzyme distribution pattern resembling that of the inflammatory hypertrophic epidermis; type 2 was similar in enzymatic pattern to the advancing proximal portion of the regenerating epidermis; and type 3 was similar enzyme-histochemically to the undifferentiated epithelium of the early embryo skin. Thus, a great variation in the distribution pattern of enzyme activities appears during the carcinogenesis process.

Submitted on June 28, 1965


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1966