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HISTONE SPECIFICITY REVEALED BY AMMONIACAL SILVER STAINING

MAURICE M. BLACK 1 and HUIDSON R. ANSLEY 1

1 Department of Pathology, New Work Medical College, Fifth Arenue and 106th Street, New York, New York 10029

Post-formalin ammoniacal-silver, a staining method which is selective for histone, imparts different colors, ranging from yellowish to black, to many different cell types. The same colors are imparted to histone extracts of the cell types in question. Pure lysine as well as so-called lysine-rich histone subfractions stain yellowish, while pure arginine and so-called arginine-rich subfractions stain black. The mechanism of the color differences of the stain is somewhat elucidated by these findings, and the study of histone specificity facilitated.

Submitted on June 28, 1965


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