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THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE

RICHARD C. GRAHAM JR. 1 and MORRIS J. KARNOVSKY 1

1 Department of Pathology, Harvard Medical School, Boston, Massachusetts

The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studied with a new ultrastructural cytochemical technique. In animals killed as early as 90 sec after injection, reaction product was found on the brushborder membranes and in the apical tubular invaginations. From the latter structures it was transported to the apical vacuoles, in which it was progressively concentrated to form protein absorption droplets. The method, which employs 3,3'-diaminobenzidine as oxidizable substrate, gives sharp localization and is sensitive. This system is advantageous in studying the early stages of renal tubular protein absorption, since small amounts of protein on membranes and in tubules and vesicles can be detected easily. The method also appears promising for studying protein transport in a variety of other cells and tissues.

Submitted on September 7, 1965


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 J. Histochem. Cytochem.Home page
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Home page
 J. Histochem. Cytochem.Home page
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[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
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Home page
 Infect. Immun.Home page
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 JCBHome page
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Home page
 JCBHome page
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Home page
 J. Cell Sci.Home page
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[Abstract] [Full Text] [PDF]

Home page
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 Am. J. Respir. Cell Mol. Bio.Home page
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[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
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J. Exp. Biol., May 15, 2003; 206(10): 1707 - 1715.
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 BloodHome page
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Home page
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[Abstract] [Full Text]

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 J. Histochem. Cytochem.Home page
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[Abstract] [Full Text]

Home page
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[Abstract] [Full Text]

Home page
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Am J Physiol Gastrointest Liver Physiol, December 1, 1998; 275(6): G1309 - G1316.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A. E. Busch, U. Karbach, D. Miska, V. Gorboulev, A. Akhoundova, C. Volk, P. Arndt, J. C. Ulzheimer, M. S. Sonders, C. Baumann, et al.
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Mol. Pharmacol., August 1, 1998; 54(2): 342 - 352.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
L. Konrad, P. Schiemann, H. Renneberg, G. Wennemuth, C. Fini, and G. Aumüller
Expression and Enzymic Activity of Ecto 5'-Nucleotidase in the Human Male Genital Tract
Biol Reprod, July 1, 1998; 59(1): 190 - 196.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
A. H.N. Hopman, F. C.S. Ramaekers, and E. J.M. Speel
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J. Histochem. Cytochem., June 1, 1998; 46(6): 771 - 778.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
P. M. Novikoff and A. Yam
Stem Cells and Rat Liver Carcinogenesis: Contributions of Confocal and Electron Microscopy
J. Histochem. Cytochem., May 1, 1998; 46(5): 613 - 626.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
M. Ueda, A. Maeda, N. Nakagawa, T. Kase, R. Kubota, H. Takakura, A. Ohshima, and Y. Okuno
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J. Clin. Microbiol., February 1, 1998; 36(2): 340 - 344.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
J. Gonatas, Y. Chen, A Stieber, Z Mourelatos, and N. Gonatas
Truncations of the C-terminal cytoplasmic domain of MG160, a medial Golgi sialoglycoprotein, result in its partial transport to the plasma membrane and filopodia
J. Cell Sci., January 1, 1998; 111(2): 249 - 260.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Ichikawa, K. Ishihara, T. Kusakabe, M. Kurihara, T. Kawakami, T. Takenaka, K. Saigenji, and K. Hotta
Distinct effects of tetragastrin, histamine, and CCh on rat gastric mucin synthesis and contribution of NO
Am J Physiol Gastrointest Liver Physiol, January 1, 1998; 274(1): G138 - G146.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. W. Gresik, M. Kashimata, Y. Kadoya, R. Mathews, N. Minami, and S. Yamashina
Expression of Epidermal Growth Factor Receptor in Fetal Mouse Submandibular Gland Detected by a Biotinyltyramide-based Catalyzed Signal Amplification Method
J. Histochem. Cytochem., December 1, 1997; 45(12): 1651 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Bendayan and E. A. Rasio
Evidence of a Tubular System for Transendothelial Transport in Arterial Capillaries of the Rete Mirabile
J. Histochem. Cytochem., October 1, 1997; 45(10): 1365 - 1378.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Ding and H. F. Voigt
Intracellular Response Properties of Units in the Dorsal Cochlear Nucleus of Unanesthetized Decerebrate Gerbil
J Neurophysiol, May 1, 1997; 77(5): 2549 - 2572.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Bakker, P Webster, W. Jacob, and N. Andrews
Homotypic fusion between aggregated lysosomes triggered by elevated [Ca2+]i in fibroblasts
J. Cell Sci., January 9, 1997; 110(18): 2227 - 2238.
[Abstract] [PDF]

Home page
 Stem CellsHome page
F Servida, D Soligo, L Caneva, F Bertolini, E de Harven, S Campiglio, C Corsini, and G. Deliliers
Functional and morphological characterization of immunomagnetically selected CD34+ hematopoietic progenitor cells
Stem Cells, July 1, 1996; 14(4): 430 - 438.
[Abstract]

Home page
 J. Biol. Chem.Home page
C. E. Futter, C. N. Connolly, D. F. Cutler, and C. R. Hopkins
Newly Synthesized Transferrin Receptors Can Be Detected in the Endosome before They Appear on the Cell Surface
J. Biol. Chem., May 5, 1995; 270(18): 10999 - 11003.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E van der Zee, V Everts, K Hoeben, and W Beertsen
Immunolocalisation of collagenase in rabbit periosteal tissue explants and extraction of the enzyme. The effect of the cytokines IL-1 alpha and EGF
J. Cell Sci., January 4, 1994; 107(4): 1047 - 1053.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
W Stoorvogel, V Oorschot, and B Neve
A novel method for measuring protein expression at the cell surface
J. Cell Sci., January 12, 1993; 106(4): 1201 - 1209.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
S. Heusser, S. Colin, A. Figiel, C. Huet, J. M. Keller, P. Pornet, S. Robine, J. Vandamme, J. Vandekerckhove, and M. Dauca
Amphibian intestinal villin: isolation and expression during embryonic and larval development
J. Cell Sci., November 1, 1992; 103(3): 699 - 708.
[Abstract] [PDF]

Home page
 ScienceHome page
D. Lefebvre, A Giaid, H Bennett, R Lariviere, and H. Zingg
Oxytocin gene expression in rat uterus
Science, June 12, 1992; 256(5063): 1553 - 1555.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
T. Sawada, T. Yamamoto, T. Yanagisawa, S. Takuma, H. Hasegawa, and K. Watanabe
Evidence for Uptake of Basement Membrane by Differentiating Ameloblasts in the Rat Incisor Enamel Organ
Journal of Dental Research, August 1, 1990; 69(8): 1508 - 1511.
[PDF]

Home page
 FAKE JDRHome page
D.F. Turner, C.F. Marfurt, and C. Sattelberg
Demonstration of Physiological Barrier Between Pulpal Odontoblasts and its Perturbation Following Routine Restorative Procedures: A Horseradish Peroxidase Tracing Study in the Rat
Journal of Dental Research, August 1, 1989; 68(8): 1262 - 1268.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
M. Jontell, M.N. Gunraj, and G. Bergenholtz
Immunocompetent Cells in the Normal Dental Pulp
Journal of Dental Research, June 1, 1987; 66(6): 1149 - 1153.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
Y. Kubo
The Uptake of Horseradish Peroxidase in Monkey Temporomandibular Joint Synovium after Occlusal Alteration
Journal of Dental Research, May 1, 1987; 66(5): 1049 - 1054.
[Abstract] [PDF]

Home page
 ScienceHome page
J. Rosenstein
Neocortical transplants in the mammalian brain lack a blood-brain barrier to macromolecules
Science, February 13, 1987; 235(4790): 772 - 774.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
A.R. Hand, R. Coleman, M.R. Mazariegos, J. Lustmann, and L.V. Lotti
Endocytosis of Proteins by Salivary Gland Duct Cells
Journal of Dental Research, February 1, 1987; 66(2): 412 - 419.
[Abstract] [PDF]

Home page
 FAKE JDRHome page
L.J. Walsh, G.J. Seymour, and N.W. Savage
Oral Mucosal Langerhans Cells Express DR and DQ Antigens
Journal of Dental Research, March 1, 1986; 65(3): 390 - 393.
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Home page
 FAKE JDRHome page
S.B. Shellhammer, J.M. Gowgiel, G.C. Gaik, and F.S. Weine
Somatotopic Organization and Transmedian Pathways of the Rat Trigeminal Ganglion
Journal of Dental Research, November 1, 1984; 63(11): 1289 - 1292.
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Home page
 ScienceHome page
J. Rosenstein and M. Brightman
Circumventing the blood-brain barrier with autonomic ganglion transplants
Science, August 26, 1983; 221(4613): 879 - 881.
[Abstract] [PDF]

Home page
 ScienceHome page
J. Hanker and B. Giammara
Neutrophil pseudoplatelets: their discrimination by myeloperoxidase demonstration
Science, April 22, 1983; 220(4595): 415 - 417.
[Abstract] [PDF]

Home page
 ScienceHome page
R. Broadwell, M Salcman, and R. Kaplan
Morphologic effect of dimethyl sulfoxide on the blood-brain barrier
Science, July 9, 1982; 217(4555): 164 - 166.
[Abstract] [PDF]

Home page
 Arch NeurolHome page
M. Iivanainen, P. O. Leinikki, E. I. Taskinen, I. C. Shekarchi, D. L. Madden, and J. L. Sever
Oligoclonal Bands in Myoclonus Epilepsy-Reply
Arch Neurol, May 1, 1982; 39(5): 321 - 321.
[Abstract] [PDF]