QUANTITATIVE ANALYSIS OF THE ESTERASES IN RAT MEGAKARYOCYTES BY A RADIO-ISOTOPE CYTOCHEMICAL TECHNIQUE
1 Molecular Enzymology Unit, Department of Biochemical Pharmacology, State University of New York, Buffalo, New York 14214
Megakaryocytes from bone marrow of immature rats have been treated with 3H-DFP, and the grain density over them measured in autoradiographs. The uptake of labeled DFP is reduced by one-third by eserine and by the AChase inhibitor 284C51, and by reactivation with pyridine-2-aldoxime. It is concluded that one-third of the DFP taken up by megakaryocytes represents binding to true AChase. The other esterases present include no significant amount of pseudocholinesterase. Submitted on December 7, 1965
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