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RESOLVING POWER, SENSITIVITY AND LATENT IMAGE FADING OF SOLUBLE-COMPOUND AUTORADIOGRAPHS

T. C. APPLETON 1

1 Medical Research Council, Biophysics Research Unit, King's College, London W.C.2

The resolving power, sensitivity and latent image fading have been studied in autoradiographs prepared by a technique that retains soluble compounds. A resolution of 2-4 µ was obtained in preparations containing 3H-thymidine and 9-13 µ in those containing 22NaCl. Results showed that there was no significant diffusion of soluble compounds. Sensitivity studies revealed that –25°C was the optimum exposure temperature. There was no significant latent image fading in preparations exposed for periods up to 330 days.

Submitted on November 10, 1965


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J. L. Gerlach and B. S. McEwen
Rat Brain Binds Adrenal Steroid Hormone: Radioautography of Hippocampus with Corticosterone
Science, March 10, 1972; 175(4026): 1133 - 1136.
[Abstract] [PDF]



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