Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MCMILLAN, P. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by MCMILLAN, P. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

DIFFERENTIAL DEMONSTRATION OF MUSCLE AND HEART TYPE LACTIC DEHYDROGENASE OF RAT MUSCLE AND KIDNEY

PAUL J. MCMILLAN 1

1 Department of Anatomy, School of Medicine, Loma Linda University, Loma Linda, California

A method for the histochemical demonstration of lactic dehydrogenase (LDH) is presented. This method utilizes a semipermeable membrane to minimize enzyme diffusion and high tetrazole concentration with a controlled reaction rate to minimize product diffusion. It was demonstrated that phenazine methosulfate could be used to make the reaction independent of endogenous diaphorase providing either a cytochrome oxidase inhibitor or strict anaerobiosis is employed. Differentiation of cells containing predominantly H-LDH from cells containing predominantly M-LDH was achieved by the use of 4 molal urea or pyruvate in the incubation medium. A preliminary report of the staining patterns observed in rat gastrocnemius and kidney indicates the usefulness of the technique. A new avenue to the study of the distribution of the LDH subunits is thus opened.

Submitted on August 15, 1966


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1967