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CYTOCHEMICAL DEMONSTRATION OF GLUTATHIONE REDUCTASE (EC 1.6.4.2) IN SACCHAROMYCES CEREVISIAE

JÜRGEN REISS 1

1 Institute of Special Botany, University of Mainz, Germany

A method for the cytochemical demonstration of glutathione reductase in unfixed yeast cells (Saccharomyces cerevisiae) is described using reduced glutathione as substrate, nicotinamide adenine dinucleotide phosphate (nicotinamide adenine dinucleotide) as coenzyme and nitro blue tetrazolium as indicator. An essential prerequisite is an exactly controlled pH level (pH 6.5) of the complete incubation medium.

Submitted on October 28, 1966


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