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A FLUORESCENT METHOD FOR THE DETECTION AND LOCALIZATION OF ZINC IN HUMAN GRANULOCYTES

GARY L. SMITH 1, ROBERT A. JENKINS 1, and JAMES F. GOUGH 1

1 Department of Zoology and Physiology, University of Wyoming, Laramie, Wyoming, and The Earl and Bessie Whedon Cancer Detection Foundation, Sheridan, Wyoming

A new cytochemical method is presented for the fluorescent demonstration of zinc in human granulocytes using 8-hydroxyquinoline. This compound is known to form fluorescent complexes with magnesium, calcium and zinc. Specificity for zinc is accomplished by adjusting the staining solution to pH 8.0 with Michaelis universal buffer. The technique is simple, rapid and much more specific for zinc than the dithizone stain.

Submitted on July 3, 1969


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