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LOCALIZATION OF beta-GALACTOSIDASES AND ACID PHOSPHATASE IN THE SMALL INTESTINAL WALL COMPARISON OF ADULT AND SUCKLING RAT

CHRISTER NORDSTRÖM 1, OTAKAR KOLDOVSKYacute 1, and ARNE DAHLQVIST 1

1 Department of Physiological Chemistry, University of Lund, and Research Department, E-Blocket, Hospital of Lund, Lund, Sweden

By horizontal sectioning of fresh frozen pieces of rat intestinal wall different parts of the villi and crypts were isolated. The sections were collected in groups, homogenized and used for enzyme analyses. Single sections for histologic examination were taken before and after each collection. A quantitative comparison of the distribution of the two intestinal beta-galactosidases, EC 3.2.1.23 (neutral and acid beta-galactosidase), and the acid phosphatase, EC 3.1.3.2, in jejunum and ileum of adult and suckling (12 days old) rats was performed. The neutral beta-galactosidase, which corresponds to the enzyme usually called lactase, was always present along the villi with the highest activities in the apical halves of the villi. This distribution profile is typical for digestion enzymes. The acid beta-galactosidase, which is chiefly a heterogalactosidase, and acid phosphatase were found to have a rather flat distribution profile with about the same activities along the villi and in the crypts. The only exception was the ileum of suckling rat, in which especially the acid beta-galactosidase showed higher activity in the villi and decreased toward the crypts. The results support the concept that the neutral beta-galactosidase is responsible for the digestion of dietary lactose, while the acid beta-galactosidase seems to have a different functional significance. That this enzyme is distributed in parallel with the acid phosphatase is consistent with the suggestion that the acid beta-galactosidase may be a lysosomal enzyme.

Submitted on December 30, 1968


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R. Fortin-Magana, R. Hurwitz, J. J. Herbst, and N. Kretchmer
Intestinal Enzymes: Indicators of Proliferation and Differentiation in the Jejunum
Science, March 20, 1970; 167(3925): 1627 - 1628.
[Abstract] [PDF]



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