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LEAD ION AND PHOSPHATASE HISTOCHEMISTRY III. THE EFFECTS OF LEAD AND ADENOSINE TRIPHOSPHATE CONCENTRATION ON THE INCORPORATION OF PHOSPHATE INTO FIXED TISSUE

ALAN S. ROSENTHAL 1, HAROLD L. MOSES 1, LOIS TICE 1, and CHARLES E. GANOTE 1

1 National Institutes of Health, Bethesda, Maryland, and the Vanderbilt University School of Medicine, Nashville, Tennessee

This communication attempts to separate and define the relationships between lead inhibition of tissue adenosine triphosphatase activity, lead. catalyzed adenosine triphosphate hydrolysis and reaction product localization when the Wachstein-Meisel reaction is applied to kidney. Using a radiochemical assay of adenosine triphosphatase activity and varying the concentration of lead nitrate or adenosine triphosphate, the quantity of phosphate bound to and released from tissue was determined. Depending on the relative concentrations of lead and adenosine triphosphate, two situations may exist. With low lead or high adenosine triphosphate concentrations, phosphate release by tissue exceeds phosphate trapped by tissue and substantial quantities of phosphate are lost to the medium. With low adenosine triphosphate or high lead concentrations more phosphate is bound in tissue than can be attributed to tissue enzyme activity. Possible explanations for these phenomenon are discussed.

Submitted on April 23, 1969


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J. W. DePierre and M. L. Karnovsky
Ecto-Enzyme of Granulocytes: 5'-Nucleotidase
Science, March 15, 1974; 183(4129): 1096 - 1098.
[Abstract] [PDF]



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