Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HAGOPIAN, M.
Right arrow Articles by SPIRO, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HAGOPIAN, M.
Right arrow Articles by SPIRO, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

CYTOCHEMICAL LOCALIZATION OF CHOLINESTERASE IN EMBRYONIC RABBIT CARDIAC MUSCLE

MARTIN HAGOPIAN 1, VIRGINIA M. TENNYSON 1, and DAVID SPIRO 1

1 Department of Pathology, New York Medical College, and Department of Pathology, Division of Neuropathology and General Pathology, Columbia University, College of Physicians and Surgeons, New York, New York 10029

Cardiac muscle of rabbit embryos from day 9 through day 18 of gestation was studied by a modification of the Koelle-Friedenwald copper thiocholine technique for the localization of cholinesterase activity. In the earlier stages of development a cholinesterase, presumed to be acetylcholinesterase from its substrate and inhibitor specificity, is found in the abundant, randomly dispersed rough surfaced endoplasmic reticulum of the myoblast. Cytochemical end product is also occasionally found in the nuclear envelope, Golgi complex and subsarcolemmal cisternae. The localization of the enzyme first in the rough surfaced endoplasmic reticulum and Golgi complex and at later stages in the sarcoplasmic reticulum is significant with regard to the differentiation of the cardiac myocyte. Furthermore, the presence of this enzyme in contractile tissue before the appearance of nerves or nerve endings demonstrates an acetylcholine-cholinesterase system of myogenic origin.

Submitted on May 22, 1969


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1970