Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by RITTER, C.
Right arrow Articles by THORELL, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by RITTER, C.
Right arrow Articles by THORELL, B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

REDUCED PYRIDINE NUCLEOTIDE ABSORPTION IN INTRACELLULAR MITOCHONDRIA OF CULTURED LIVER CELLS

CARL RITTER 1 and BO THORELL 1

1 Laboratory of Pharmacology, Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, and Department of Pathology, Karolinska Sjukhuset, Stockholm, Sweden

In the 310-360 mµ range, reduced pyridine nucleotide in mitochondria which are inside human liver tissue culture cells shows an absorption maximum between 335 and 330 mµ. Thus, between one-third and two-thirds of the reduced pyridine nucleotide is protein-bound. Accompanying the changes at 330 and 335 mµ, there is a decrease in absorption at 265 mµ. The decrease in 265 mµ absorption which accompanies pyridine nucleotide reduction is more than would be expected based on the increase in absorption seen at 330-340 mµ. The extra decrease in 265 mµ absorption may have its origin in adenine nucleotide loss or decreased mitochondrial light scattering. A consideration of changes in 265 mµ absorption in cultured ascites tumor cells produced by glucose and by Dicumarol has led to the proposal that the main factor responsible for the large decrease in 265 mµ absorption is adenine nucleotide loss.

Submitted on May 16, 1969


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1970