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THE BIOCHEMICAL GENETICS OF RABBIT ERYTHROCYTE ESTERASES HISTOCHEMICAL CLASSIFICATION

ROBERT SCHIFF 1

1 Department of Veterinary Microbiology, University of California, Davis, California 95616

Esterases found in rabbit erythrocytes and platelets were separated by zone electrophoresis in starch gels. Activity tests in various substrates were used to classify the esterase bands into four major categories. The bands of each category were further subdivided into groups by the effects of various inhibiting and activating agents. These experiments showed that the genetic loci which control the enzymes of systems 1, 2 and 3 control isozymes that respond identically with the test reagents in their respective systems. Diisopropylfluorophosphate and eserine sulfate inhibition indicated that the active sites of systems 1 and 2 enzymes are different. This supported the conclusion that the esterases of systems 1 and 2 are the products of closely linked loci and not multiple alleles at a single locus. Carbonic anhydrases which migrated in the cathodal portion of the gel were identified by their sensitivity to acetazolamide and more intense staining with beta- rather than agr-naphthyl acetate as substrate.

Submitted on April 14, 1970


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