Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KERPEL-FRONIUS, S.
Right arrow Articles by HAJÓS, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KERPEL-FRONIUS, S.
Right arrow Articles by HAJÓS, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ELECTRON MICROSCOPIC DEMONSTRATION OF ENERGY PRODUCTION AND COUPLED RESPIRATION OF IN SITU MITOCHONDRIA

S. KERPEL-FRONIUS 1 and F. HAJÓS 1

1 Department of Anatomy, University Medical School, Budapest, Hungary

Respiratory-linked accumulation of Sr2+ was applied to visualize mitochondrial energy production in unfixed slices of liver, kidney and heart muscle. The reaction was dependent on the function of the respiratory chain and energy coupling as was shown by the inhibitory effect of KCN and 2,4-dinitrophenol. The electron-dense granular or needle-like reaction product was localized in the inner compartment of the swollen mitochondria. Sr2+ translocation was also utilized to detect the coupled oxidation of different respiratory substrates. The overwhelming majority of mitochondria showed, more or less, activity when malate was oxidized. In the kidney and heart muscle a few mitochondria, however, did not show succinate-supported Sr2+ accumulation. This points to the possible differences in substrate utilization of mitochondria localized in the same cells. In the liver all mitochondria exhibited marked reaction with both substrates.

Submitted on February 10, 1970


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1970