Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ESSNER, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ESSNER, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

OBSERVATIONS ON HEPATIC AND RENAL PEROXISOMES (MICROBODIES) IN THE DEVELOPING CHICK

EDWARD ESSNER 1

1 Division of Cytology, Sloan-Kettering Institute for Cancer Research, Rye, New York 10580

Peroxisomes (microbodies) in developing liver and metanephric kidney of the chick were studied with a staining method that visualizes these organelles by light and electron microscopy. In both tissues nucleoid and anucleoid peroxisomes were evident at all stages studied (15th day of incubation to the adult). Hepatic peroxisomes showed no special topologic relationship to glycogen areas and were mainly confined to other areas of the cytoplasm. Nucleoid type peroxisomes were more apparent before and after hatching and rarely observed in the adult. Peroxisomes were present in kidney proximal tubule cells, but absent from cells of distal convoluted tubules. They appeared to increase in number during development and were most conspicuous in the adult. The structure of the nucleoid in renal peroxisomes resembled that of the hepatocyte but contained a more distinct series of parallel dense laminae. Diaminobenzidine (DAB) oxidative activity, although relatively weak, visualized nucleoid and anucleoid peroxisomes in liver and kidney at all stages of development. Anucleoid peroxisomes could be distinguished from various dense bodies in hepatocytes and from droplets in proximal convoluted tubule cells, both of which lacked DAB oxidative activity. The DAB reaction in peroxisomes was not suppressed in the absence of hydrogen peroxide or in the presence of the catalase inhibitor, 3-amino-1,2,4 triazole. DAB reaction, sensitive to potassium cyanide, was localized to cristae, but not to the outer membrane, of hepatic and renal mitochondria. Mitochondria of distal tubule cells showed a stronger reaction. The possible significance of nucleoid-bearing peroxisomes in the developing chick is discussed in terms of nitrogen metabolism, uric acid excretion and the presence of the uricolytic enzymes. The nature of the DAB reaction in peroxisomes and in mitochondria is considered.

Submitted on September 11, 1969


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
K. Johkura, N. Usuda, Y. Liang, and A. Nakazawa
Immunohistochemical Localization of Peroxisomal Enzymes in Developing Rat Kidney Tissues
J. Histochem. Cytochem., October 1, 1998; 46(10): 1161 - 1174.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1970