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PERCHLORIC ACID EXTRACTION OF DEOXYRIBONUCLEIC ACID FROM THIN SECTIONS OF EPON-ARALDITE-EMBEDDED MATERIAL

WILLIAM H. J. DOUGLAS 1

1 Division of Biological and Medical Sciences, Brown University, Providence, Rhode Island 02912

A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the polytene chromosomes from Drosophila salivary glands. Chromosome bands were also rendered electron-transparent when hydroxypropylmethacrylate-embedded thin sections were digested with deoxyribonuclease or extracted with perchloric acid. Nucleolar ribonucleic acid was unaffected by the perchloric acid extraction.

Submitted on February 28, 1970


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