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UPTAKE OF 3H-NOREPINEPHRINE BY FLUORESCENT NERVES OF THE HEART

DAVID T. MASUOKA 1 and GIAN FRANCO PLACIDI 1

1 Neuropharmacology and Psychopharmacology Research Laboratories, Veterans Administration Hospital, Sepulveda, California 91343, Medical Pharmacology and Therapeutics, University of California, Irvine, California 92664

The localization of uptake 5 minutes after the injection of a low dose of 3H-norepinephrine by adrenergic nerve fibers in the heart has been observed by combining autoradiography with the formaldehyde-induced histochemical fluorescence method for aromatic monoamines in the same tissue section. The distribution of 3H-label corresponds exactly to that of the fluorescent nerves. Furthermore, uptake studies in animals depleted of catecholamines by the tyrosine hydroxylase inhibitor (H 44/68), which does not block uptake, indicate that the dosage of 3H-norepinephrine used, under the conditions of the present experiment, was not in itself sufficient to produce the intense formaldehyde-induced fluorescence in nerves. It may be concluded that 3H-norepinephrine was taken up only by those nervous structures in the heart which show a specific fluorescence reaction due to the presence of endogenous catecholamine.

Submitted on June 11, 1969


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