Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by QUINTARELLI, G.
Right arrow Articles by ZITO, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by QUINTARELLI, G.
Right arrow Articles by ZITO, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ON PHOSPHOTUNGSTIC ACID STAINING. II

G. QUINTARELLI 1, J. A. CIFONELLI 2, and R. ZITO 3

1 The University of Alabama Medical Center, Birmingham, Alabama 35233 and the Regina Elena Institute for Cancer Research, Rome, Italy
2 The Department of Pediatrics and Biochemistry, La Rabida-University of Chicago Institute, Chicago, Illinois 60637
3 The Regina Elena Institute for Cancer Research, Rome, Italy

Phosphotungstic acid (PTA) used at different concentrations in water was combined with various compounds (amino acids, proteins, acid glycosaminoglycans, glycoproteins and simple sugars). The results obtained indicated that PTA interacts with positively charged groups, that the sugar-hydroxyls do not take part in the interaction and that the type of binding involves electrostatic forces. To substantiate these findings further, heparin and heparin oligosaccharides were N-desulfated, combined with PTA and the precipitates were analyzed. It was found that the interaction had taken place only between the metal and the desulfated hexosamine amino groups and that when the N-desulfated product was acetylated no PTA binding occurred. When PTA was used at higher concentrations and in extremely acidic conditions, a precipitation of proteins, amino acids, acid glycosaminoglycans and neutral sugars ensued. This complex formation was interpreted as due to ionic interaction between the polyacid and the protonated hydroxyl groups.

Submitted on May 19, 1971


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1971