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AMINOACRIDINE DYES AND THE SECONDARY STRUCTURE OF DNA IN SITU

MANUEL DIAZ 1, JOSE HIERRO 2, and GRACIELA DEMICHELI DE DIAZ 3

1 Laboratorio de Genética, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay
2 Laboratorio de Genética, Facultad de Agronomía, and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
3 Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay

A new method is proposed to study the secondary structure of deoxyribonucleic acid (DNA) in situ in fixed chromatin. It is based on acriflavine staining and on differentiation with a nitrous acid solution of the fixed cytologic preparation. The presence of green fluorescence after this treatment is regarded as indicative of double stranded DNA. Experiments are described with DNA-acriflavine mixtures in solution, DNA-agar models and cytologic preparations submitted to different pretreatments. The feasibility and limitations of the method are discussed in the light of the results reported upon.

Submitted on August 11, 1971


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