Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MEDNICK, M. L.
Right arrow Articles by SELIGMAN, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MEDNICK, M. L.
Right arrow Articles by SELIGMAN, A. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

LOCALIZATION OF ACETYLCHOLINESTERASE VIA PRODUCTION OF OSMIOPHILIC POLYMERS: NEW BENZENEDIAZONIUM SALTS WITH THIOLACETATE FUNCTIONS

MORTON L. MEDNICK 1, JOHN P. PETRALI 1, NORMAN C. THOMAS 1, LUDWIG A. STERNBERGER 1, ROBERT E. PLAPINGER 1, DONALD A. DAVIS 1, HANNAH L. WASSERKRUG 1, and ARNOLD M. SELIGMAN 1

1 Basic Sciences Department, Medical Research Laboratory, Edgewood Arsenal, Maryland 21210, and Department of Surgery, Sinai Hospital of Baltimore 21215, and Departments of Microbiology and Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21215

By incorporating an enzyme-susceptible thiolester group and a diazonium group into the same molecule, it has been possible to obtain an osmiophilic polymer upon enzymatic hydrolysis of the thiolester, via diazothioether formation linking the units. The thiolacetates with diazonium groups are specific substrates for cholinesterase because of the strong positive charge on the diazonium group. Following osmication, sites of acetylcholinesterase in motor end plate are notable as black deposits in light microscopy and opaque deposits in electron microscopy. This is the first cytochemical demonstration of a hydrolytic enzyme using a single agent as both substrate and capture reagent to form a polymer. The insolubility of the polymer in both water and lipid before osmication provides precision of localization needed for the high resolution of electron microscopy. However, the effectiveness of the new principle for electron microscopy depends as well upon the rate of the capture reaction (polymerization), which in turn depends upon the pKa of the thiol and pH and temperature at which the cytochemical reaction is conducted.

Submitted on September 22, 1970


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1971