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ELECTRON STAINING WITH URANYL ACETATE POSSIBLE ROLE OF FREE AMINO GROUPS

LUCIANO LOMBARDI 1, GIOVANNI PRENNA 2, LAJOS OKOLICSANYI 3, and ALAIN GAUTIER 4

1 Instituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy
2 Centro di Studio per l'Istochimica del CNR, Pavia, Italy
3 Istituto di Patologia Speciale Medica dell'Universita, Padova, Italy
4 Centre de Microscopie, Electronique de l'Université, Lausanne, Switzerland

Uranyl acetate (UA) is known to have affinity for nucleic acids and –COOH groups. Experiments were performed to test the possible affinity of UA for free amino groups. For this purpose, –NH2 blocking and deamination techniques (with dinitrofluorobenzene, nitrous acid and Chloramine T) were applied to form-aldehyde-fixed pancreas samples, after which the stainability of zymogen granules by UA was strongly reduced. It is concluded that free amino groups may also participate in the binding of UA in biologic specimens.

Submitted on June 24, 1970


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