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VISUALIZATION IN FREEZE-DRIED TISSUE SECTIONS OF STRUCTURES TO BE STUDIED BY QUANTITATIVE HISTOCHEMISTRY

BO HELLMAN 1 and ÅKE LERNMARK 1

1 Department of Histology, University of Umeå, 90187 Umeå 6, Sweden

The limiting factor for quantitative histochemistry employing freeze-dried tissue sections is the ability to identify morphologic structures. Satisfactory staining of freeze-dried tissue specimens intended for further microanalysis was achieved by using isopentane solutions of free dye bases. The practical details of this rapid histologic control are outlined and its usefulness is illustrated by measurements of fructose 1,6-diphosphate and insulin in microdissected specimens of pancreatic beta cells.

Submitted on December 7, 1970


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