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LOCALIZATION OF ENDOGENOUS PEROXIDASE IN RAT EXORBITAL LACRIMAL GLAND

EDWARD ESSNER 1

1 Division of Cytology, Sloan-Kettering Institute for Cancer Research, New York, New York 10021

Cytochemical localization of endogenous peroxidase was studied in the exorbital lacrimal gland of the rat. By light microscopy, reaction product (oxidized diaminobenzidine) was observed in the basal cytoplasm of acinar cells, in many secretory granules and in a smaller population of granules, sometimes distributed around the periphery of the secretory material. By electron microscopy, peroxidase reaction product was localized in all cisternae of the rough endoplasmic reticulum including the perinuclear cisternae, in a population of electron-opaque granules that resembled lysosomes and in secretory granules. Small, opaque, well preserved secretory granules were peroxidase-positive; larger, less opaque, poorly preserved granules were peroxidase-negative. In Golgi areas near positively stained secretory granules, reaction product was present in one, possibly two, Golgi saccusles and in condensing vacuoles of various sizes. In some intercalated duct cells, reaction product was localized in endoplasmic reticulum and in a few small granules. The results suggest that rough endoplasmic reticulum, Golgi apparatus and condensing vacuoles are involved in the synthesis and segregation of peroxidase, which is finally stored in some, but perhaps not all, secretory granules. A possible role for lacrimal gland peroxidase and its relationship to the lactoperoxidase of salivary and mammary glands are discussed.

Submitted on November 16, 1970


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