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A CYTOPHOTOMETRIC STUDY OF THE DEOXYRIBONUCLEIC ACID-FEULGEN DYE CONTENT AND AREA OF HUMAN GRANULOCYTES AND LYMPHOCYTES

PHYLLIS GOTTLIEB-ROSENKRANTZ 1 and REGINA O'BRIEN 1

1 IBM Watson Laboratory at Columbia University, New York, New York 10027

Human granulocytes and lymphocytes, fixed in formalin and stained according to the Feulgen reaction, were measured microspectrophotometrically with the two-wavelength method. Deoxyribonucleic acid (DNA)-Feulgen dye content and nuclear area were determined. Significant differences in DNA-Feulgen dye content between gransulocytes and lymphocytes occurred in most cases regardless of hydrolysis time, but the magnitude of these differences varied from one blood sample to another. A significant positive correlations between DNA-Feulgen dye content of optimally hydrolyzed nuclei and their areas has beens shows for both granulocytes and lymphocytes. These findings are discussed in the context of the "compaction" hypothesis.

Submitted on April 14, 1970


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