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HIGH RESOLUTION AUTORADIOGRAPHY WITH STRIPPING FILM

JOSEPH R. WILLIAMSON 1 and HANK VAN DEN BOSCH 1

1 Departments of Pathology and Biological Chemistry, Washington University School of Medicine, St. Louis, Missouri 63110

A simple method is described for the preparation and use of stripping film of high quality for electron microscopic applications of autoradiography. The advantages of the method are: (a) uniform monolayers of emulsion are easily prepared with an inexpensive mechanical device and applied to thin sections on conventional grids; (b) both sides of grids can be coated with emulsion, permitting a potential doubling of sensitivity with, theoretically, no loss of resolution; and (c) large numbers of grids can be processed simultaneously, assuring identical conditions of development.

Submitted on January 18, 1971


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