Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by NOVIKOFF, A. B.
Right arrow Articles by QUINTANA, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by NOVIKOFF, A. B.
Right arrow Articles by QUINTANA, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

STUDIES ON MICROPEROXISOMES II. A CYTOCHEMICAL METHOD FOR LIGHT AND ELECTRON MICROSCOPY

ALEX B. NOVIKOFF 1, PHYLLIS M. NOVIKOFF 1, CLEVELAND DAVIS 1, and NELSON QUINTANA 1

1 Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, New York 10461

A modification of the Novikoff-Goldfischer alkaline 3,3'-diaminobenzidine medium for visualizing peroxisomes is described. It makes possible light microscopic as well as electron microscopic studies of a recently described class of peroxisomes, the microperoxisomes. Potassium cyanide (5 x 10–3 M) is included in the medium to inhibit mitochondrial staining, the pH is 9.7 and there is a high concentration of H2O2 (0.05%). Two cell types have been chosen to illustrate the advantages of the new procedure for demonstrating the microperoxisomes: the absorptive cells in the human jejunum and the distal tubule cells in the guinea pig kidney. Suggestive relations of microperoxisomes and lipid are described in the human jejunum. The microperoxisomes are strategically located between smooth endoplasmic reticulum that radiates toward the organelles and contains lipid droplets and "central domains" of highly specialized endoplasmic reticulum which do not show the lipid droplets. The microperoxisomes are also present at the periphery of large lipid-like drops. In the guinea pig kidney tubule there is a striking difference between the thick limb of Henle and distal tubule. The distal tubule has a population of cells with large numbers of microperoxisomes readily visible by light microscopy; these cells are not present in the thick limb of Henle. Other differences between the two are also described.

Submitted on June 29, 1972


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Saito-Nakano, T. Yasuda, K. Nakada-Tsukui, M. Leippe, and T. Nozaki
Rab5-associated Vacuoles Play a Unique Role in Phagocytosis of the Enteric Protozoan Parasite Entamoeba histolytica
J. Biol. Chem., November 19, 2004; 279(47): 49497 - 49507.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H Voigt, J. Olivo, P Sansonetti, and N Guillen
Myosin IB from Entamoeba histolytica is involved in phagocytosis of human erythrocytes
J. Cell Sci., January 4, 1999; 112(8): 1191 - 1201.
[Abstract] [PDF]

Home page
 J. Histochem. Cytochem.Home page
P. M. Novikoff and A. Yam
Stem Cells and Rat Liver Carcinogenesis: Contributions of Confocal and Electron Microscopy
J. Histochem. Cytochem., May 1, 1998; 46(5): 613 - 626.
[Abstract] [Full Text]

Home page
 Toxicol PatholHome page
H. Maruyama, T. Tanaka, and G. M. Williams
Effects of the Peroxisome Proliferator Di(2-Ethylhexyl)Phthalate on Enzymes in Rat Liver and on Carcinogen-Induced Liver Altered Foci in Comparison to the Promoter Phenobarbital
Toxicol Pathol, February 1, 1990; 18(2): 257 - 267.
[Abstract] [PDF]

Home page
 ScienceHome page
P. Lazarow
Three hypolipidemic drugs increase hepatic palmitoyl-coenzyme A oxidation in the rat
Science, August 5, 1977; 197(4303): 580 - 581.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1972

 
Purchase HCS Short Course Manual on HCS site