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A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF AMINOPEPTIDASE B ACTIVITY

KAUKO K. MÄKINEN 1 and KEIJO U. PAUNIO 1

1 Institute of Dentistry, University of Turku, SF-20520 Turku 52, Finland

A histochemical method for the demonstration of aminopeptidase B activity is presented. The method is based on the specific activation of the enzyme by chloride ions present in physiologic concentrations (0.9% or 0.154 M) in the incubating medium, on the inhibition by phosphate ions and on the use of N-l-arginyl-2-naphthylamine as substrate. The liberated 2-naphthylamine was coupled with fast blue B.

Submitted on June 28, 1971


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