ESTERASE ISOZYMES FROM RABBIT SYNOVIAL FLUIDS NORMAL AND ARTIFICIAL JOINTS
1 Departments of Oral Surgery, Tufts University Schools of Medicine and Dental Medicine, Boston, Massachusetts 02111
Acetyl- and butrylesterase isozymes from knee and temporomandibular joint synovial fluid, serum and red cells of New Zealand White rabbits were compared by starch gel electrophoresis to the isozymes obtained from a false joint fluid produced in three animals by interposing a sheet of Silastic between segments of a fracture of the left subcondylar area. All fluids studied had two major regions of esterase activity that migrated at rates comparable to systems 1 and 2 of the red cell. However, inhibition tests differentiated the body fluid esterases from those of the red cell. Knee, temporomandibular joint and false joint fluids had similar zymograms. Analysis of the false joint fluid reinforced light microscopic findings that not only does a synovial membrane differentiate de novo but the membrane produces or maintains a fluid similar in esterase activity to the normal temporomandibular joint. Submitted on August 30, 1971
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