Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by WESTE, S. M.
Right arrow Articles by PENINGTON, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by WESTE, S. M.
Right arrow Articles by PENINGTON, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

FLUOROMETRIC MEASUREMENT OF DEOXYRIBONUCLEIC ACID IN BONE MARROW CELLS THE MEASUREMENT OF MEGAKARYOCYTE DEOXYRIBONUCLEIC ACID

SANDRA M. WESTE 1 and DAVID G. PENINGTON 1

1 University of Melbourne Department of Medicine, St. Vincent's Hospital, Fitzroy, Victoria, 3065, Australia

The use of fluorochromes in the Feulgen reaction for the quantitative measurement of deoxyribonucleic acid (DNA) has been explored previously but is rarely applied. The occurrence of nonspecific fluorescence due to compounds other than DNA and the fading of the fluorochrome on excitation can cause variation in the measurements of nuclear fluorescence. The method of Ruch (in Introduction to Quantitative Cytochemistry, Wied, G. L., ed., Academic Press, 1966, p. 281) using auramine O as the fluorochrome has been modified to achieve improved nuclear fluorescence with a more linear rate of fading. A simple Zeiss microscope photometer has been used. Problems of instrumentation and optics, including lens changes during measurements on cells of widely varying sizes such as megakaryocytes, have been analyzed and correction factors have been calculated. Microfluorometric measurements of nuclear DNA are of the same order of accuracy as those made by integrating microdensitometry, without the need in the latter technique to compress large cells to a uniform thickness. Microfluorometry has been applied with the new staining method to analyze the ploidy distribution of bone marrow megakaryocytes.

Submitted on December 20, 1971


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1972