Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LEEMANN, U.
Right arrow Articles by RUCH, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LEEMANN, U.
Right arrow Articles by RUCH, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

CYTOFLUOROMETRIC DETERMINATION OF BASIC AND TOTAL PROTEINS WITH SULFAFLAVINE

U. LEEMANN 1 and F. RUCH 1

1 Department of General Botany, Swiss Federal Institute of Technology, Zürich, Switzerland

The cytofluorometric determination of histones and total proteins with a single acid dye, Sulfaflavine, at the pHs 8.0 and 2.8, is described. Successive staining and measuring at the two pHs allow the determination of histones and total proteins in individual cells. Optimal staining conditions were determined; the specificity and the linearity of the staining were confirmed in model experiments (protein smears) and with different kinds of cells. The protein smears also served for calibration in absolute terms; the obtained protein values agreed with those calculated from dry weight measurements and with values from the literature. The influence of fixation (formalin and ethanol-acetic acid) and preparation, the reproducibility of the staining reaction and the inherent limitations of the method are discussed.

Submitted on November 22, 1971


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1972