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STANDARDS FOR ELECTRON PROBE MICROANALYSIS OF BIOLOGIC SPECIMENS

G. M. LEHRER 1 and C. BERKLEY 1

1 Division of Neurochemistry, Department of Neurology, Mount Sinai School of Medicine of the City University of New York, New York, New York 10029

A procedure is described for the preparation and application of gelatin standards in the quantitative elemental analysis of microscopic portions of tissue sections in the electron microprobe x-ray spectrometer. Ten to fifteen per cent gelatin solutions containing varying concentrations of the elements to be measured are quick-frozen in capsules, sectioned in a cryostat at the same thickness as the tissue and freeze-dried. Standard sections are mounted with each tissue section, carbon-coated and analyzed simultaneously. Concentrations of the elements in portions of the tissue as small as 10–12 liters are then determined directly from a regression curve or equation derived from the standards.

Submitted on May 18, 1972


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