Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by BRODY, M. J.
Right arrow Articles by SUNDLER, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by BRODY, M. J.
Right arrow Articles by SUNDLER, F.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

CELLULAR LOCALIZATION OF GLUCAGON BY FLUORESCENCE MICROSCOPY REACTION OF NH2-TERMINAL HISTIDINE WITH o-PHTHALDIALDEHYDE

M. J. BRODY 1, R. HAKANSON 1, I. LUNDQUIST 1, C. OWMAN 1, and F. SUNDLER 1

1 Departments of Pharmacology and Histology, University of Lund, Lund, Sweden

Exposure to o-phthaldialdehyde (OPT) causes intense fluorescence in A2-cells in sections from pancreas. Alloxan treatment of mice was followed by a marked increase in the number and fluorescence intensity of the OPT-reactive A2-cells, whereas cobalt chloride given to guinea pigs induced an almost total depletion of the cytoplasmic fluorescence with concomitant reduction of their argyrophilia. Microspectrofluorometric analysis of the fluorophore revealed excitation maxima at 370 and 420 mµ; the corresponding emission maxima were 430 and 490 mµ. This closely resembled the spectral characteristics of the OPT-induced fluorescence of authentic glucagon. It is concluded that OPT forms fluorphores wth glucagon in the A2-cells and that the NH2-terminal histidine residue of the polypeptide is responsible for the formation of the fluorophore. It is proposed that OPT is a useful reagent for the histochemical detection not only of histamine but also of histidyl peptides.

Submitted on July 17, 1972


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1973