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HISTOCHEMICAL LOCALIZATION OF ADENYL CYCLASE IN THE FUNGUS PHYCOMYCES BLAKESLEEANUS

J. C. TU 1 and S. K. MALHOTRA 1

1 Biological Sciences Electron Microscopy Laboratory, University of Alberta, Edmonton, Alberta, Canada T6G 2E9

The presence of adenyl cyclase in Phycomyces blakesleeanus has been investigated in situ histochemically by lead precipitation of pyrophosphate liberated by conversion of adenosine triphosphate to cyclic adenosine monophosphate. Dormant spores, heat-shocked spores, germinating spores and the growing zone of sporangiophore (stage IV) of the fungus were studied. Electron-dense lead precipitate was localized in association with plasma membrane and between the mitochondrial membranes and nuclear membranes in all stages investigated. This reaction product is not inhibited by sodium fluoride, and adenyl cyclase is the only known enzyme stimulated by sodium fluoride. Comparable studies on the hepatocytes of mouse liver showed the reaction product in association with plasma membrane only.

Submitted on April 24, 1973


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